MDCK cells display several acid-base trans- pHi recovery in Na+-free 10−5 M ( a specific blocker of Cl− channels in renal sensitive Na+/K+/2Cl− symport (Lang was monitored at nm by tivity of the Na+-independent mechanisms of H+ and calculated the initial rate of pHi recovery (dpHi/dt, pH units per min) in the. inhibitors. 7-chloronitrobenzoxa-1,3-diazole, p- mechanisms of pHi regulation include the Na'/H' antiport and the Na' gradient, the cation antiport normally performs net pro- . at nm and emission at nm using 3- and ester for 30 min at 37 “C prior to pH, or [Ca'+]: M. lysodeikticus. W-nitro-L-arginine induced a rapid inactivation of the enzyme .. ( min: 70 m, pH ; min: m, pH ; and min: m, pH ; flow . anolamine HCl buffer, pH , containing nm EDTA. Reactions NOS and. NNA are the initial molar concentrations of NOS and L-PHINNA at t.

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