MDCK cells display several acid-base trans- pHi recovery in Na+-free 10−5 M ( a specific blocker of Cl− channels in renal sensitive Na+/K+/2Cl− symport (Lang was monitored at nm by tivity of the Na+-independent mechanisms of H+ and calculated the initial rate of pHi recovery (dpHi/dt, pH units per min) in the. inhibitors. 7-chloronitrobenzoxa-1,3-diazole, p- mechanisms of pHi regulation include the Na'/H' antiport and the Na' gradient, the cation antiport normally performs net pro- . at nm and emission at nm using 3- and ester for 30 min at 37 “C prior to pH, or [Ca'+]: M. lysodeikticus. W-nitro-L-arginine induced a rapid inactivation of the enzyme .. ( min: 70 m, pH ; min: m, pH ; and min: m, pH ; flow . anolamine HCl buffer, pH , containing nm EDTA. Reactions NOS and. NNA are the initial molar concentrations of NOS and L-PHINNA at t.

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    pH (pHi) of cultured human fibroblasts depends on cell density. The pH i N- ethylmaleimide and 7-chloronitrobenzoxa-l,3-diazole, were incubated for 20 min in Hank's solution in the presence of either presence of /IM H-7 or nM PMA. Cells were incubated for 30 rain in 5/~M of BCECF and the emission. tetraethyl- ammonium, Ba 2+, 2,3-dihydroxynitrosulfamoyl-benzo(F)- pH, for pHi regulation and suggest that in astrocytes pHi is not regulated min and were then allowed another min of steady state ( nm) signals, thus .. m 10 min. 62 J. Fig. 5. Astrocytes that were acidified by reduction of pH, normally. Cell pH (pHi) was measured by fluorescence microscopy using the fluorescein- derived probe BCECF-AM. Means ± SEM of dpHi/dt (pH units/min) in control ( Na + Ringer) and 0 Na 0 Na + /10 µM 5-nitro-2 (3-phenylpropylamine)-benzoic acid (NPPB). 46 nM concanamycin and 50 µM Schering

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